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KMID : 0380019950100040468
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 4 p.468 ~ p.473
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Study of Enzyme Immobilization on Composite of CTA and PCL Membrane for Biosensor
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Abstract
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The disposable glucose biosensor using composite of CTA and PCL membrane was developed for measurement of glucose. The most effective membrane was composed of CTA/PCL(80/20, w/w) and, glutaraldehyde one-step immobilization method (10um thickness) for glucose sensor gave the best result among various methods coidi
,nserng oxygen permeability and electronic sensitivity. A scanning elec
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tron micrograph of the cross-section of a typical asymmetric CTA/PCL composite membrane showed that the membrane fused with a dense layer covered with a GOD-glutaraldehyde. Glucose oxidase immoblilized on the membrane showed the linearity between difference of absolute amperometric values and glucose concentrations within 7mM when the GOD immobilized electrode was used. About 35% of activity was remained after 8 days when the tyrosinase was immobilized on CTA/PCL (80/20) membrane.
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